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1 Product Result. | Match Criteria: Keyword. MAC132. clone 4G10.1, from mouse. Sigma-Aldrich. pricing.

Dmtmm sigma

For DMTMM-coupled reactions, 450 pmoles of DNA was combined with peptide (270 nmoles, 600 eq.) and DMTMM (4.5 μmoles, 10,000 eq.). EDC-based coupling reactions followed the same procedure as above using EDC (4.5 μmoles, 10,000 eq) and N-hydroxy Frankfurt, Germany), trehalose (Sigma-Aldrich, Steinheim, Germany) and glycerol (Sigma-Aldrich, Steinheim, Germany) were used. As a coupling agent, 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chlo-ride (DMTMM, Sigma Aldrich, Steinheim, Germany) was employed. For in vitro cell experiments HEp-2 cells (ATCC, grade, purchased from Sigma Aldrich (Buchs, Switzerland) and used as received. 2.2.

Janus cykliska peptid-polymer nanorör - naturkommunikation

Propylphosphonic anhydride (T3P ® ) is a very efficient, nontoxic coupling reagent especially suited for connecting sterically hindered amino acids. A further advantage is the easy removal of byproducts by extraction of the crude product with water.

Compact Quantum Dots for Single-molecule Imaging - JoVE

The formation of pGlu in proteins, similar to Suc, Human serum (Sigma-Aldrich, H4522) was centrifuged at 13000 rpm for 10 min to remove lipids before use. For DMTMM-coupled reactions, 450 pmoles of DNA was combined with peptide (270 nmoles, 600 eq.) and DMTMM (4.5 μmoles, 10,000 eq.). EDC-based coupling reactions followed the same procedure as above using EDC (4.5 μmoles, 10,000 eq) and N-hydroxy Frankfurt, Germany), trehalose (Sigma-Aldrich, Steinheim, Germany) and glycerol (Sigma-Aldrich, Steinheim, Germany) were used. As a coupling agent, 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chlo-ride (DMTMM, Sigma Aldrich, Steinheim, Germany) was employed. For in vitro cell experiments HEp-2 cells (ATCC, grade, purchased from Sigma Aldrich (Buchs, Switzerland) and used as received. 2.2.

oC and analyzed by MALDI MS. Masses of the reaction products for the light (H8) form of the reagent are: 217 – free Ac-Arg; 241 – DMTMM; 373 – dead-end Ac-Arg crosslink; 571 – inter-Ac-Arg crosslink.
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have developed DMTMM BF 4 1b as an alternative to 1a. 7 The non-nucleophilic BF 4-counterion does not take part in the degradation process and organic solutions of 1b are stable for several days at least.

Degradation of DMTMM was fol-lowedat37 Cindeuteriumoxideforupto48h.Aseriesof1H-NMR spectra were acquired on a Bruker Avance AV-500 NMR spec- DMTMM (3.3 mg, 0.012 mmol) was added to the ZW800-1C solution, and the mixture was stirred at room temperature for 30 min. 1,4-diaminobutane (15.4 mg, 0.18 mmol) in 1 mL of DW was added dropwise ORIGINAL CONTRIBUTION Conformational study on the thermal transition of chitosan-g-poly(N-vinylcaprolactam) in aqueous solution Daniel Fernández-Quiroz1 & Álvaro González-Gómez2,3 & Jaime For DMTMM-coupled reactions, 450 pmoles of DNA was combined with peptide (270 nmoles, 600 eq.) and DMTMM (4.5 μmoles, 10,000 eq.). EDC-based coupling reactions followed the same procedure as above using EDC (4.5 μmoles, 10,000 eq) and N-hydroxy Our study demonstrates the advantages of DMTMM conjugation as a powerful tool to cal grade, purchased from Sigma Aldrich (Buchs, Switzerland) and.
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Janus cykliska peptid-polymer nanorör - naturkommunikation

The reaction was maintained at room temperature for 2 hours. To remove unreacted DMTMM and AEM, the Using degassed pH 6.5 2-(N-morpholino)ethanesulfonic acid (MES)-buffered saline (MBS) with 10 mM ethylenediaminetetraacetic (EDTA) acid as a buffer, PGA stocks were prepared at 13.2 mg/mL (100 mM glutamate monomer). 4-(4,6-Dimethoxy-1,3,5-triazin-2-yl)–4-methylmorpholinium tetrafluoroborate (DMTMM; Sigma-Aldrich Co., St Louis, MO, USA) was added at 150 mol% relative to glutamate … 2019-08-26 immobilized on DEAE Sepharose media (DFF-100, Sigma). Reactions with water-soluble coupling reagents EDC (1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochlo-ride) and DMTMM (4-(4,6-dimethoxy[1,3,5]triazin-2-yl)-4-methyl-morpholinium chloride) were completed using both Sepharose media and in solution-phase.

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Compact Quantum Dots for Single-molecule Imaging - JoVE

obtained from Sigma-Aldrich were kept in the dark. A commercially available linear poly(ε-caprolactone) (PCL) with Mw ~80 000 g/mol (CAPA 6806) was supplied by Perstorp and used as received. Dimethylsufloxide (DMSO), dichloromethane (DCM), toluene and dimethylformamide (DMF) were purchased from Sigma-Aldrich and distilled prior to their use. DMTMM chemistry resulted effective also in absence of pH control, which is essential for EDC/NHS conjugation.